apraga/org - Change I4J2NZJXY644RCHEMPLTWC7PBY53BQAWBUJQXTRBUXPLLRI4R3IQC

Spectrophotométrie

Created by Alexis Praga on July 2, 2024

I4J2NZJXY644RCHEMPLTWC7PBY53BQAWBUJQXTRBUXPLLRI4R3IQC

Dependencies

In channels

main

Change contents

Insertion in projects.org at line 253 [4.179]

[2.420]

[3.524]

** TODO Mettre en place prélèvement loyer
SCHEDULED: <2024-07-02 Tue>
/Entered on/ [2024-07-02 Tue 22:54]

Insertion in projects.org at line 306 [4.179]

[5.2182]

** TODO Envoyer remboursement podologue mutuelle
SCHEDULED: <2024-07-02 Tue>
/Entered on/ [2024-07-02 Tue 22:54]

File addition: 20240702233311-triglycerides.org (----------)

[6.188]

:PROPERTIES:
:ID:       f7fa1113-bc2f-4ca2-b99f-eb4a7f4ed346
:END:
#+title: Triglycérides
#+filetags: biochimie
Interférences : glycérole (excès), vitamine C (défaut)
Normes 1.70mmol/L

File addition: 20240702233233-cholesterole_ldl.org (----------)

[6.188]

:PROPERTIES:
:ID:       3093198d-a9d2-4464-a453-9b6bffb03841
:END:
#+title: Cholestérole LDL
#+filetags: biochimie
Interférence :triglycériduqe > 33.9mmol/L, cholestérol > 12.9mmol/L
Normes 2.6mmol/L

File addition: 20240702233106-cholesterol_hdl.org (----------)

[6.188]

:PROPERTIES:
:ID:       e5519b58-b8fd-4ae1-9585-aa5223d72431
:END:
#+title: Cholestérol HDL
#+filetags: biochimie
Interférence : triglycéride > 4.5mmol/L
Normes 1.04-1.55mmol/L

File addition: 20240702233046-cholesterol_total.org (----------)

[6.188]

:PROPERTIES:
:ID:       b2e9a998-da30-4b85-8f7d-6af80ce3c059
:END:
#+title: Cholestérol total
#+filetags: biochimie
Norme < 5.20mmol/L

File addition: 20240702232836-acides_biliaires.org (----------)

[6.188]

:PROPERTIES:
:ID:       b86a1b39-27b0-43e3-8630-7840234e9c71
:END:
#+title: Acides biliaires
#+filetags: biochimie
Augmént cholesases
Attention à la toxicité cardiaque foetale au 3e trimestre de la grossesse
Normes 0-10μmol/L

File addition: 20240702232812-ethanol.org (----------)

[6.188]

:PROPERTIES:
:ID:       4e543bc3-7e7e-4f29-a0f2-898f4539b8e5
:END:
#+title: Éthanol
#+filetags: biochimie
Indication: intoxication aigüe
Norme < 0.03g/L

File addition: 20240702232724-bicarbonates.org (----------)

[6.188]

:PROPERTIES:
:ID:       b29858f9-80d4-409e-b006-ca8c80c3e849
:END:
#+title: Bicarbonates
#+filetags: biochimie
CO2 + H2O -> HCO3- + H+
Évalue la réserve alcaline
Interférence : lactescence > 30g/L
Norme 21-32mmol/L

File addition: 20240702232642-acide_lactique.org (----------)

[6.188]

:PROPERTIES:
:ID:       714ee729-bccd-4465-88df-04b8824f5098
:END:
#+title: Acide lactique
#+filetags: biochimie
Évalue état de choc (hypoxie métabolique)
Normes 0.4-2mmol/L

File addition: 20240702232550-glucose.org (----------)

[6.188]

:PROPERTIES:
:ID:       48f25a79-0610-4804-b404-8d3aefdb918e
:END:
#+title: Glucose
#+filetags: biochimie
Interférence urinaire : métronidazole
Normes
- sérum 4.07-5.83mmol/L
- urines < 0.5g/24h
- LCR 0.4-0.7g/L

File addition: 20240702232452-ldh.org (----------)

[6.188]

:PROPERTIES:
:ID:       d3c7d1e8-2219-4be5-86e1-34c6dbc77a70
:END:
#+title: LDH (lactase déshydrogénase)
#+filetags: biochimie
Marqueur lyse cellulaire (non spécifique d'un tissu car 5 isonezyme)
Interférence : hémolyse > 10mg/dL, EDTA, fluorure, Na, oxalate, potassium
Normes 100-200UI/L

File addition: 20240702232402-creatine_kinase.org (----------)

[6.188]

:PROPERTIES:
:ID:       0286b98f-eb1f-41f0-80c5-ecfe06e243da
:END:
#+title: Créatine kinase
#+filetags: biochimie
Marqueur lyse musculaire/cardiaque/cérébrale
Interférence : B12
Normes 20-230 UI/L

File addition: 20240702231944-ammoniac.org (----------)

[6.188]

:PROPERTIES:
:ID:       977fa2d7-2989-4f7a-afa4-6be072722c41
:END:
#+title: Ammoniac
#+filetags: biochimie
Évalue la capacité de détoxification azoté hépatique
Norme < 32μmol/L

File addition: 20240702231447-uree.org (----------)

[6.188]

:PROPERTIES:
:ID:       5e7c2de5-958a-4d37-8796-4f18d45df53b
:END:
#+title: Urée
#+filetags: biochimie
Interférence NH(+)
Normes 2.5-6.5

File addition: 20240702230555-α_amylase.org (----------)

[6.188]

:PROPERTIES:
:ID:       d70072d4-776f-4b80-8df7-0d2526ca269d
:END:
#+title: α-Amylase
#+filetags: biochimie
Diagnostic parotitide
Interférence : protéines > 120g/L, IgG > 50g/L, triglycérides > 30g/L
Normes 20-120 UI/L

File addition: 20240702230440-lipase.org (----------)

[6.188]

:PROPERTIES:
:ID:       c691319f-47cc-4586-86c6-121dae677abf
:END:
#+title: Lipase
#+filetags: biochimie
Diagnostic de pancréatite
Interférence EDTA, oxalate, fluorure, citrate
Normes 70-400UI/L

File addition: 20240702230347-γgt_gammaglutamyl_transferase.org (----------)

[6.188]

:PROPERTIES:
:ID:       08f57dfb-caed-4cba-ac90-f0bcffcdb7b4
:END:
#+title: γGT (gammaglutamyl-transférase)
#+filetags: biochimie
Marqueur hépatopathies
Sensible mais non spécifique
Interférence : oxalate, potassium, fluorure, sodium
Normes 10-70UI/L

File addition: 20240702230230-phosphatase_alcalines.org (----------)

[6.188]

:PROPERTIES:
:ID:       257d79ff-411a-4ba7-83ed-459856b5d7fa
:END:
#+title: Phosphatase alcaline
#+filetags: biochimie
marqueur cholestase hépatique (défaut écoulement biliaire)
isoenzyme : foie, os, intestin, placenta
interférence : annuler si lactescence > 400mg/dL
norme 45-120 UI/L

File addition: 20240702230203-acide_urique.org (----------)

[6.188]

:PROPERTIES:
:ID:       0cd6155a-54ba-4718-a639-67944c6b1c47
:END:
#+title: Acide urique
#+filetags: biochimie
dégradation des purines
interférence (diminution) : xanthine (caféine, théine)
normes homme 200-450μol/L, femme 150-350μmol/L

File addition: 20240702230001-proteines_urinaires_lcr.org (----------)

[6.188]

:PROPERTIES:
:ID:       6b2862c8-b11d-44d7-b5a4-abd1272b6e86
:END:
#+title: Protéines urinaires/LCR
#+filetags: biochimie
normes
- urines <150mg/24h
- LCR 150-450mg/L

File addition: 20240702225936-proteines_seriques_totales.org (----------)

[6.188]

:PROPERTIES:
:ID:       5ca3120e-340e-4228-9d79-8111105208f7
:END:
#+title: Protéines sériques totales
#+filetags: biochimie
diag : pathologie foie, reins, troubles métaobliques/nutritionnels
normes 64-82g/L

File addition: 20240702225917-phosphore_inorganique.org (----------)

[6.188]

:PROPERTIES:
:ID:       bab652dd-fa28-4dba-941d-7e4b32902525
:END:
#+title: Phosphore inorganique
#+filetags: biochimie
interférences défaut lipides > 30g/L, mannitol, excès phosphate de créatinine, pyruvate de phosphoénol
normes 0.8-1.6

File addition: 20240702225843-magnesium.org (----------)

[6.188]

:PROPERTIES:
:ID:       bec091db-6da1-46ad-8c31-c55e551fbc36
:END:
#+title: Magnésium
#+filetags: biochimie
Norme 0.75-1mmol/L

File addition: 20240702225415-fer.org (----------)

[6.188]

:PROPERTIES:
:ID:       1aa04c29-ad9a-48e0-9392-73a8a5deee65
:END:
#+title: Fer
#+filetags: biochimie
norme
- homme 10-30μmol/L
- femme 8-28μmol/L

File addition: 20240702225354-creatinine.org (----------)

[6.188]

:PROPERTIES:
:ID:       97cfbabe-78e8-49e8-8b00-01e47a6f2da9
:END:
#+title: Créatinine
#+filetags: biochimie
Norme 53-115

File addition: 20240702225333-calcium.org (----------)

[6.188]

:PROPERTIES:
:ID:       e618c88d-3a3a-483a-bb75-30dde2738c01
:END:
#+title: Calcium
#+filetags: biochimie
Norme 2.12-2.52 mmol/L

Replacement in notes/20240630235344-spectrophotometrie.org at line 26 [2.1261]
B:BD[2.2026] → [2.2026:2051]
```
* Dosages en point final
```
[2.2026]
[2.2051]
```
* Dosage colorimétrique en point final
```

Insertion in notes/20240630235344-spectrophotometrie.org at line 29 [2.1261]

[2.2165]

- [[id:e618c88d-3a3a-483a-bb75-30dde2738c01][Calcium]]
- [[id:97cfbabe-78e8-49e8-8b00-01e47a6f2da9][Créatinine]]
- [[id:1aa04c29-ad9a-48e0-9392-73a8a5deee65][Fer]]
- [[id:bec091db-6da1-46ad-8c31-c55e551fbc36][Magnésium]]
- [[id:bab652dd-fa28-4dba-941d-7e4b32902525][Phosphore inorganique]]
- [[id:5ca3120e-340e-4228-9d79-8111105208f7][Protéines sériques totales]]
- [[id:6b2862c8-b11d-44d7-b5a4-abd1272b6e86][Protéines urinaires/LCR]]

Replacement in notes/20240630235344-spectrophotometrie.org at line 37 [2.1261]

B:BD[2.2166] → [2.2166:2168]

[2.2166]

* Dosage enzymatique en cinétique
** Absorbance propre
- [[id:0cd6155a-54ba-4718-a639-67944c6b1c47][Acide urique]]
** Clivé par l'enzyme à doser libérant un colorant
- [[id:257d79ff-411a-4ba7-83ed-459856b5d7fa][Phosphatase alcalines]]
- [[id:08f57dfb-caed-4cba-ac90-f0bcffcdb7b4][γGT (gammaglutamyl-transférase)]]
- [[id:c691319f-47cc-4586-86c6-121dae677abf][Lipase]]
- [[id:d70072d4-776f-4b80-8df7-0d2526ca269d][α-Amylase]]
** Substrat réagissant avec une enzyme utilisant le cofacteur NADH
Mesure 340nm
- [[id:5e7c2de5-958a-4d37-8796-4f18d45df53b][Urée]]
- [[id:977fa2d7-2989-4f7a-afa4-6be072722c41][Ammoniac]]
- [[id:90cbcd7e-9606-47b8-ad0b-2c4d38996b1b][Transaminases (ASAT, ALAT)]]
- [[id:0286b98f-eb1f-41f0-80c5-ecfe06e243da][Créatine kinase]]
- [[id:d3c7d1e8-2219-4be5-86e1-34c6dbc77a70][LDH]]
- [[id:48f25a79-0610-4804-b404-8d3aefdb918e][Glucose]]
- [[id:714ee729-bccd-4465-88df-04b8824f5098][Acide lactique]]
- [[id:b29858f9-80d4-409e-b006-ca8c80c3e849][Bicarbonates]]
- [[id:4e543bc3-7e7e-4f29-a0f2-898f4539b8e5][Éthanol]]
- [[id:b86a1b39-27b0-43e3-8630-7840234e9c71][Acides biliaires]]
** Réaction de Trinder
Réaction aboutissant à formation H2O2 qui va catalyse la synthèse d'un olorant
Interférence: ce qui dégrade le H2O2 (acide ascorbique (vitamine C), dopamine

Insertion in notes/20240608183004-transaminases_asat_alat.org at line 5 [3.1657]
[7.179]
[3.1761]
Replacement in notes/20240608183004-transaminases_asat_alat.org at line 9 [3.1657]
B:BD[3.2018] → [7.269:301]
```
- ALAT (alanine transférase) :
```
[3.2018]
[7.301]
```
- ALAT (alanine transférase) : marqueur cytolyse hépatique
```
Replacement in notes/20240608183004-transaminases_asat_alat.org at line 12 [3.1657]
B:BD[7.464] → [7.464:498]
```
- ASAT (aspartate transférase) :
```
[7.464]
[7.498]
```
- ASAT (aspartate transférase) : cytolyse hépatocyte ou cardiomyocyte
```
Deletion in notes/20240608183004-transaminases_asat_alat.org at line 25 [3.1657]
B:BD[3.2615] → [3.2615:2616]

Deletion in notes/20240608183004-transaminases_asat_alat.org at line 29 [3.1657]

B:BD[3.2673] → [3.2673:2756]

B:BD[3.2756] → [7.1070:1187]


* Principales interactions analytiques
- hémolyse
- gammapathie IgM (très rare)
- ALAT:
  -hydroxycobalamine (vitamine B12) (diminution)
  - sulfasalazine (anti-inflammatoire intestinal: Crohn...)

Replacement in notes/20240608183004-transaminases_asat_alat.org at line 30 [3.1657]

B:BD[3.2826] → [3.2826:2952]

* Vigilance pré-analytiques et analytiques
- Prélèvement non hémolysé
- tube hépariné (lithium, di- ou tri-potassique)

[3.2826]

[3.2952]

* Interférence
ALAT: B12, isoniazide
ASAT: hémolyse (annulé si hémolyse > 25mg/dL)

Insertion in notes/20221104120759-biochimie.org at line 14 [10.1130]

[8.7127]

[9.4426]

- [[id:0286b98f-eb1f-41f0-80c5-ecfe06e243da][Créatine kinase]]
- [[id:02953d37-3891-43b1-9e07-cb4681e5e44c][Bilirubine]]
- [[id:08f57dfb-caed-4cba-ac90-f0bcffcdb7b4][γGT (gammaglutamyl-transférase)]]
- [[id:0aebd019-c8f1-4f88-8a6b-94e06eb14f64][Acides aminés]]
- [[id:0cd6155a-54ba-4718-a639-67944c6b1c47][Acide urique]]

Insertion in notes/20221104120759-biochimie.org at line 20 [10.1130]

[9.4486]

- [[id:1aa04c29-ad9a-48e0-9392-73a8a5deee65][Fer]]
- [[id:20192370-bbe2-476b-beab-9eedb5bf243c][Immunoglobuline M]]
- [[id:257d79ff-411a-4ba7-83ed-459856b5d7fa][Phosphatase alcalines]]

Insertion in notes/20221104120759-biochimie.org at line 24 [10.1130]
[9.4564]
[9.4564]
```
- [[id:3093198d-a9d2-4464-a453-9b6bffb03841][Cholestérole LDL]]
```

Insertion in notes/20221104120759-biochimie.org at line 26 [10.1130]

[9.4627]

- [[id:48f25a79-0610-4804-b404-8d3aefdb918e][Glucose]]
- [[id:4e543bc3-7e7e-4f29-a0f2-898f4539b8e5][Éthanol]]
- [[id:5ca3120e-340e-4228-9d79-8111105208f7][Protéines sériques totales]]
- [[id:5e7c2de5-958a-4d37-8796-4f18d45df53b][Urée]]
- [[id:6653dbbb-6de9-4386-905d-c1e82b3b448e][Immunoglobuline G]]
- [[id:6b2862c8-b11d-44d7-b5a4-abd1272b6e86][Protéines urinaires/LCR]]

Insertion in notes/20221104120759-biochimie.org at line 33 [10.1130]

[9.4693]

- [[id:714ee729-bccd-4465-88df-04b8824f5098][Acide lactique]]
- [[id:90cbcd7e-9606-47b8-ad0b-2c4d38996b1b][Transaminases (ASAT, ALAT)]]

Insertion in notes/20221104120759-biochimie.org at line 37 [10.1130]

[9.4806]

- [[id:977fa2d7-2989-4f7a-afa4-6be072722c41][Ammoniac]]
- [[id:97cfbabe-78e8-49e8-8b00-01e47a6f2da9][Créatinine]]

Insertion in notes/20221104120759-biochimie.org at line 41 [10.1130]

[9.4918]

- [[id:b29858f9-80d4-409e-b006-ca8c80c3e849][Bicarbonates]]
- [[id:b2e9a998-da30-4b85-8f7d-6af80ce3c059][Cholestérol total]]

Replacement in notes/20221104120759-biochimie.org at line 44 [10.1130]

B:BD[9.4974] → [9.4974:5034]

B:BD[9.5034] → [2.4507:4637]

- [[id:d9c7eb89-3d73-4fa9-a734-9dd71515cc91][Haptoglobine]]
- [[id:6653dbbb-6de9-4386-905d-c1e82b3b448e][Immunoglobuline G]]
- [[id:20192370-bbe2-476b-beab-9eedb5bf243c][Immunoglobuline M]]

[9.4974]

[2.4637]

- [[id:b86a1b39-27b0-43e3-8630-7840234e9c71][Acides biliaires]]
- [[id:bab652dd-fa28-4dba-941d-7e4b32902525][Phosphore inorganique]]
- [[id:bec091db-6da1-46ad-8c31-c55e551fbc36][Magnésium]]
- [[id:c691319f-47cc-4586-86c6-121dae677abf][Lipase]]
- [[id:d3c7d1e8-2219-4be5-86e1-34c6dbc77a70][LDH]]
- [[id:d70072d4-776f-4b80-8df7-0d2526ca269d][α-Amylase]]

Replacement in notes/20221104120759-biochimie.org at line 51 [10.1130]

∅:D[2.4702] → [9.5034:5035]

B:BD[9.5034] → [9.5034:5035]

∅:D[9.5035] → [8.7127:7189]

B:BD[8.7127] → [8.7127:7189]

B:BD[8.7366] → [8.7366:7439]


- [[id:0aebd019-c8f1-4f88-8a6b-94e06eb14f64][Acides aminés]]
- [[id:90cbcd7e-9606-47b8-ad0b-2c4d38996b1b][Transaminase (ASAT, ALAT)]]

[2.4702]

- [[id:d9c7eb89-3d73-4fa9-a734-9dd71515cc91][Haptoglobine]]
- [[id:e5519b58-b8fd-4ae1-9585-aa5223d72431][Cholestérol HDL]]
- [[id:e618c88d-3a3a-483a-bb75-30dde2738c01][Calcium]]
- [[id:f7fa1113-bc2f-4ca2-b99f-eb4a7f4ed346][Triglycérides]]

Spectrophotométrie

Dependencies

In channels

Change contents

Insertion in projects.org at line 253 [4.179]

Insertion in projects.org at line 306 [4.179]

File addition: 20240702233311-triglycerides.org (----------)

File addition: 20240702233233-cholesterole_ldl.org (----------)

File addition: 20240702233106-cholesterol_hdl.org (----------)

File addition: 20240702233046-cholesterol_total.org (----------)

File addition: 20240702232836-acides_biliaires.org (----------)

File addition: 20240702232812-ethanol.org (----------)

File addition: 20240702232724-bicarbonates.org (----------)

File addition: 20240702232642-acide_lactique.org (----------)

File addition: 20240702232550-glucose.org (----------)

File addition: 20240702232452-ldh.org (----------)

File addition: 20240702232402-creatine_kinase.org (----------)

File addition: 20240702231944-ammoniac.org (----------)

File addition: 20240702231447-uree.org (----------)

File addition: 20240702230555-α_amylase.org (----------)

File addition: 20240702230440-lipase.org (----------)

File addition: 20240702230347-γgt_gammaglutamyl_transferase.org (----------)

File addition: 20240702230230-phosphatase_alcalines.org (----------)

File addition: 20240702230203-acide_urique.org (----------)

File addition: 20240702230001-proteines_urinaires_lcr.org (----------)

File addition: 20240702225936-proteines_seriques_totales.org (----------)

File addition: 20240702225917-phosphore_inorganique.org (----------)

File addition: 20240702225843-magnesium.org (----------)

File addition: 20240702225415-fer.org (----------)

File addition: 20240702225354-creatinine.org (----------)

File addition: 20240702225333-calcium.org (----------)

Replacement in notes/20240630235344-spectrophotometrie.org at line 26 [2.1261]

Insertion in notes/20240630235344-spectrophotometrie.org at line 29 [2.1261]

Replacement in notes/20240630235344-spectrophotometrie.org at line 37 [2.1261]

Insertion in notes/20240608183004-transaminases_asat_alat.org at line 5 [3.1657]

Replacement in notes/20240608183004-transaminases_asat_alat.org at line 9 [3.1657]

Replacement in notes/20240608183004-transaminases_asat_alat.org at line 12 [3.1657]

Deletion in notes/20240608183004-transaminases_asat_alat.org at line 25 [3.1657]

Deletion in notes/20240608183004-transaminases_asat_alat.org at line 29 [3.1657]

Replacement in notes/20240608183004-transaminases_asat_alat.org at line 30 [3.1657]

Insertion in notes/20221104120759-biochimie.org at line 14 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 20 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 24 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 26 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 33 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 37 [10.1130]

Insertion in notes/20221104120759-biochimie.org at line 41 [10.1130]

Replacement in notes/20221104120759-biochimie.org at line 44 [10.1130]

Replacement in notes/20221104120759-biochimie.org at line 51 [10.1130]